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Cereal Chem 46:181 - 188.  |  VIEW ARTICLE
Starch-Complexed Lipids: Differences in Extraction with Various Solvents.

S. Rogols, J. E. Green, and M. A. Hilt. Copyright 1969 by the American Association of Cereal Chemists, Inc. 

The yield and composition of various starch-complexed lipids depend on the choice of solvent used in the extraction. This was observed when the lipids present in starch were quantitated by two different extraction techniques, in one of which carbon tetrachloride was used and in the other, ether. Fractionation on silicic acid columns by the technique of Hirsch and Ahrens (J. Biol. Chem. 233: 311; 1958) has shown additional differences in the components of the extracted lipids. These may be sufficient to account for the discrepancies that appear when starch-complexed lipids are quantitated. This is substantiated by silicic-acid chromatography of both the phospholipid and the glyceride fractions. Marked differences in nature of the lipid material were observed, indicating possible dependence on the polarity of the extracting solvent.

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